the first report of cmy, aac (6′)-ib and 16s rrna methylase genes among pseudomonas aeruginosa isolates from iran

conclusions: this study detected multiple drug resistance in pseudomonas aeruginosa including resistance to β-lactams, aminoglycosides, and fluoroquinolones. therefore, identification of drug resistance patterns in p. aeruginosa and detection of pan-resistant producing isolates are of great importance in prevention and control of infections in burn center ward. background: serious infections by pseudomonas aeruginosa are commonly treated with the combination of a beta-lactam antibacterial and an aminoglycoside. therefore, production of a 16s rrna methylase may result in an extremely important antibacterial resistance profile. objectives: the present study was conducted to determine the prevalence of cephamycinase (cmy), aminoglycoside 6'-n-acetyltransferase (aac(6′)-ib) and 16s rrna methylase genes among pseudomonas aeruginosa isolates from iran. patients and materials: this descriptive study was performed on hospitalized burnt patients during 2011and 2012. antibiotics susceptibility tests were performed by disc diffusion and broth microdilution methods. cmy, aac(6′)-ib, 16s rrna methylase genes were detected by pcr method. results: seventy-seven (77%) of 100 isolates were resistant to imipenem and ceftazidime. aac(6)-ib, cephamycinase (cmy), and rrna methyltransferase (rmtb and rmtd) were detected in 57 (74.02%), 7 (9.09%), 11 (14.28%), and 9 (11.68%) isolates, respectively. pcr results for aminoglycoside resistance methyltransferase (arma) and rrna methyltransferase (rmtc) were all negative. aminoglycoside resistance methyltransferase (arma), and rrna methyltransferase (rmtc) were not detected.